ACHE Polyclonal Antibody (CAB2806)

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General Information
Description OverviewTitle:ACHE Polyclonal Antibody (CAB2806)Size:100 μLCode:CAB2806Host Species:RabbitPurification:Affinity purificationIsotype:IgGBackgroundAcetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. Immunogen InformationImmunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 398-617 of human ACHE (NP_056646.1).GeneID:43SwissProt:P22303Synonyms:ACHE; ACEE; ARACHE; N-ACHE; YT; acetylcholinesteraseCalculated MW:58kDa/65kDa/67kDaObserved MW:75kDaApplicationsReactivity:Human, Mouse, RatTested Applications:WB; IHCRecommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100Storage Buffer:Store at -20 °C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Application Key:Western blotting Immunohistochemistry Positive Samples:SH-SY5Y, SGC-996, Mouse brain, Mouse spinal cord, Mouse liver, Rat spinal cordCellular Location:Cell junction, Cell membrane, Extracellular side, GPI-anchor, Lipid-anchor, Nucleus, Peripheral membrane protein, Secreted, synapseAntibody ImagesWestern blot analysis of extracts of various cell lines, using ACHE antibody (CAB2806) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.Immunohistochemistry of paraffin-embedded mouse brain using ACHE antibody (CAB2806) at dilution of 1:100 (40x lens). Datasheets

Product Information

Purchasing Information
Certification RUO
French code (NACRES) NA.41
UNSPSC code 12352203
EU customs code 30021010
Vendor country IE
Human origin No
Uniprot P22303
Brand Reagent Genie
Product properties
Supplier category 2 Neuroscience
Supplier category 1 Antibody Genie
Supplier category 3 ANTIBODY
Marketing brochures
Clonality Polyclonal
Host Rabbit
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 398-617 of human ACHE (NP_056646.1).
Isotype (antibody class) IgG
Validated reactivity Human , Mouse , Rat
Technical presentation
KO validated No
Shelf-life (months) 12
Shipping temperature +4°C
Dilution for application WB 1:500 - 1:2000
Storage temperature +4°C
Applications WB (Western Blot) , IHC (ImmunoHistoChemistry)
Research area Neuroscience

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