ENC1 Polyclonal Antibody (CAB10257)

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General Information
Description OverviewTitle:ENC1 Polyclonal Antibody (CAB10257)Size:100 μLCode:CAB10257Host Species:RabbitPurification:Affinity purificationIsotype:IgGBackgroundThis gene encodes a member of the kelch-related family of actin-binding proteins. The encoded protein plays a role in the oxidative stress response as a regulator of the transcription factor Nrf2, and expression of this gene may play a role in malignant transformation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Immunogen InformationImmunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ENC1 (NP_003624.1).GeneID:8507SwissProt:O14682Synonyms:ENC1; CCL28; ENC-1; KLHL35; KLHL37; NRPB; PIG10; TP53I10; ectodermal-neural cortex 1Calculated MW:57kDa/66kDaObserved MW:66kDaApplicationsReactivity:Human, Mouse, RatTested Applications:WBRecommended Dilution:WB 1:1000 - 1:2000Storage Buffer:Store at -20 °C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Application Key:Western blotting Positive Samples:Jurkat, U-87MG, SW480, MCF-7, HT-1080, Mouse brain, Rat brainCellular Location:Cytoplasm, Nucleus matrix, cytoskeletonAntibody ImagesWestern blot analysis of extracts of various cell lines, using ENC1 antibody (CAB10257) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 40s. Datasheets

Product Information

Purchasing Information
Certification RUO
French code (NACRES) NA.41
UNSPSC code 12352203
EU customs code 30021010
Vendor country IE
Human origin No
Target Details
Other names ENC1 , CCL28 , ENC-1 , KLHL35 , KLHL37 , NRPB , PIG10 , TP53I10 , ectodermal-neural cortex 1
MW target protein (kDa) 57kDa/66kDa
Uniprot O14682
Brand Assay Genie
Product properties
Supplier category 2 Cell Biology
Supplier category 1 Antibody Genie
Supplier category 3 ANTIBODY
Marketing brochures https://yris.ozyme.fr/reg-brochure
Clonality Polyclonal
Host Rabbit
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ENC1 (NP_003624.1).
Isotype (antibody class) IgG
Validated reactivity Human , Mouse , Rat
Technical presentation https://www.antibodygenie.com/content/Technical%20Manuals/CAB10257.pdf
KO validated No
Shelf-Life (months) 12
Shipping temperature +4°C
Dilution for application WB 1:1000 - 1:2000
Storage temperature +4°C
Applications WB (Western Blot)
Research area Cell Biology

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