Human DPYD / Dihydropyrimidine Dehydrogenase ELISA Kit

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HUFI02398

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General Information
Description Human DPYD / Dihydropyrimidine Dehydrogenase ELISA Kit - InformationThe ELISA Genie DPYD / Dihydropyrimidine Dehydrogenase ELISA Kit can assay for DPYD / Dihydropyrimidine Dehydrogenase in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.How our DPYD / Dihydropyrimidine Dehydrogenase ELISA Kits Work? The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate. At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3',5,5'-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound DPYD / Dihydropyrimidine Dehydrogenase is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.Human DPYD / Dihydropyrimidine Dehydrogenase ELISA Kit - Data Description Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil. Post-Translational Modification Uniprot ID Q12882 Alias DPYD/Dihydropyrimidine Dehydrogenase Detection method Sandwich ELISA Double Antibody Application This immunoassay kit allows for the in vitro quantitative determination of DPYD concentrations in serum plasma and other biological fluids. Size 96T Range 0.156-10ng/ml Sensitivity < 0.094ng/ml Storage 4'C for 6 months Recovery Matrices listed below were spiked with certain level of DPYD and the recovery rates were calculated by comparing the measured value to the expected amount of DPYD in samples. Matrix Recovery range(%) Average(%) serum(n=5) 93-100 97 EDTA plasma(n=5) 94-104 97 UFH plasma(n=5) 93-102 99 Linearity The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DPYD and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample 1:2 1:4 1:8 1:16 serum(n=5) 85-101% 91-99% 87-104% 96-103% EDTA plasma(n=5) 88-100% 85-96% 84-100% 92-101% UFH plasma(n=5) 87-99% 82-100% 80-92% 80-97% CV(%) Intra-Assay: CV<8% Inter-Assay: CV<10% Note For Research Use Only

Product Information

Purchasing Information
Certification RUO
French code (NACRES) NA.84
UNSPSC code 41116126
EU customs code 30021010
Human origin No
Vendor country IE
Brand
Brand Assay Genie
Product properties
Supplier category 2 Human ELISA Kit
Supplier category 1 ELISA Genie
Supplier category 3 ELISA
Marketing brochures https://yris.ozyme.fr/reg-brochure
Product presentation https://yris.ozyme.fr/reg-elisa-precoates-genie
Technical presentation https://www.elisagenie.com/content/FI%20Product%20DataSheets/HUFI02398.pdf
Usage
Shelf-life (months) 06 , 6
Shipping temperature +4°C
Storage temperature +4°C
Applications ELISA

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