Human IGIP / IgA-inducing protein homolog ELISA Kit

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HUFI02120

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General Information
Description Human IGIP / IgA-inducing protein homolog ELISA Kit - InformationThe Human IGIP / IgA-inducing protein homolog ELISA Kit can assay for IGIP / IgA-inducing protein homolog in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.How our IGIP / IgA-inducing protein homolog ELISA Kits Work? The ELISA Genie ELISA (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, We have developed a range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with IGIP / IgA-inducing protein homolog. During the reaction, IGIP / IgA-inducing protein homolog in the sample or standard competes with a fixed amount of IGIP / IgA-inducing protein homolog on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to IGIP / IgA-inducing protein homolog. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IGIP / IgA-inducing protein homolog in the samples is then determined by comparing the O.D. of the samples to the standard curve.Human IGIP / IgA-inducing protein homolog ELISA Kit - Data Description Enhances IgA secretion from B-cells stimulated via CD40. Post-Translational Modification Uniprot ID A6NJ69 Alias IGIP Detection method CompetitiveCompetitive Application This immunoassay kit allows for the in vitro quantitative determination of IGIP concentrations in serum plasma and other biological fluids. Size 96T Range 0.312-20ng/ml Sensitivity < 0.188ng/ml Storage 4'C for 6 months Recovery Matrices listed below were spiked with certain level of IGIP and the recovery rates were calculated by comparing the measured value to the expected amount of IGIP in samples. Matrix Recovery range(%) Average(%) serum(n=5) 87-104 94 EDTA plasma(n=5) 87-104 95 UFH plasma(n=5) 90-102 95 Linearity The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IGIP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample 1:2 1:4 1:8 1:16 serum(n=5) 92-103% 96-101% 90-99% 87-100% EDTA plasma(n=5) 82-101% 84-97% 82-95% 86-99% UFH plasma(n=5) 80-99% 85-100% 88-100% 80-98% CV(%) Intra-Assay: CV<8% Inter-Assay: CV<10% Note For Research Use Only

Product Information

Purchasing Information
Certification RUO
French code (NACRES) NA.84
UNSPSC code 41116126
EU customs code 30021010
Human origin No
Vendor country IE
Brand
Brand Assay Genie
Product properties
Supplier category 2 Human ELISA Kit
Supplier category 1 ELISA Genie
Supplier category 3 ELISA
Marketing brochures https://yris.ozyme.fr/reg-brochure
Product presentation https://yris.ozyme.fr/reg-elisa-precoates-genie
Technical presentation https://www.elisagenie.com/content/FI%20Product%20DataSheets/HUFI02120.pdf
Usage
Shelf-Life (months) 06 , 6
Shipping temperature +4°C
Storage temperature +4°C
Applications ELISA

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