Human MMP17 ELISA Kit

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HUFI00720

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General Information
Description

Human MMP17 ELISA Kit - InformationThe ELISA Genie MMP17 ELISA Kit can assay for MMP17 in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.How our MMP17 ELISA Kits Work? The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate. At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3',5,5'-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound MMP17 is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.Human MMP17 ELISA Kit - Data Description Endopeptidase that degrades various components of the extracellular matrix, such as fibrin. May be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators, such as tumor necrosis factor-alpha. May also be involved in tumoral process. Cleaves pro-TNF-alpha at the '74-Ala-|-Gln-75' site. Not obvious if able to proteolytically activate progelatinase A. Does not hydrolyze collagen types I, II, III, IV and V, gelatin, fibronectin, laminin, decorin nor alpha1-antitrypsin. Post-Translational Modification The precursor is cleaved by a furin endopeptidase. Uniprot ID Q9ULZ9 Alias MMP-17/MMP17/MT4-MMP/matrix metallopeptidase 17(membrane-inserted)/matrix metalloproteinase-17/membrane-type matrix metalloproteinase 4/membrane-type-4 matrix metalloproteinase/MT4MMP/MT-MMP 4/MTMMP4 Detection method Sandwich ELISA Double Antibody Application This immunoassay kit allows for the in vitro quantitative determination of MMP17 concentrations in serum plasma and other biological fluids. Size 96T Range 0.156-10ng/ml Sensitivity < 0.094ng/ml Storage 4'C for 6 months Recovery Matrices listed below were spiked with certain level of MMP17 and the recovery rates were calculated by comparing the measured value to the expected amount of MMP17 in samples. Matrix Recovery range(%) Average(%) serum(n=5) 88-101 93 EDTA plasma(n=5) 91-103 98 UFH plasma(n=5) 89-103 96 Linearity The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MMP17 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample 1:2 1:4 1:8 1:16 serum(n=5) 99-103% 85-94% 91-105% 86-103% EDTA plasma(n=5) 83-101% 82-100% 82-101% 86-98% UFH plasma(n=5) 84-100% 81-95% 84-95% 81-99% CV(%) Intra-Assay: CV<8% Inter-Assay: CV<10% Note For Research Use Only

Product Information

Purchasing Information
Certification RUO
French code (NACRES) NA.84
UNSPSC code 41116126
EU customs code 30021010
Human origin No
Vendor country IE
Brand
Brand Assay Genie
Product properties
Supplier category 1 ELISA Genie
Product presentation https://yris.ozyme.fr/reg-elisa-precoates-genie
Marketing brochures https://yris.ozyme.fr/reg-brochure
Supplier category 3 ELISA
Supplier category 2 Human ELISA Kit
Technical presentation https://www.elisagenie.com/content/FI%20Product%20DataSheets/HUFI00720.pdf
Usage
Shelf-Life (months) 6 , 06
Shipping temperature +4°C
Storage temperature +4°C
Applications ELISA

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