Human Serpin A6 / Corticosteroid-binding globulin ELISA Kit

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General Information
Description Human Serpin A6 / Corticosteroid-binding globulin ELISA Kit - InformationThe ELISA Genie Serpin A6 / Corticosteroid-binding globulin ELISA Kit can assay for Serpin A6 / Corticosteroid-binding globulin in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.How our Serpin A6 / Corticosteroid-binding globulin ELISA Kits Work? The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate. At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3',5,5'-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound Serpin A6 / Corticosteroid-binding globulin is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.Human Serpin A6 / Corticosteroid-binding globulin ELISA Kit - Data Description Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species. Post-Translational Modification N-glycosylated; binds 5 oligosaccharide chains. Glycosylation in position Asn-260 is needed for steroid binding. Uniprot ID P08185 Alias CBG/SERPINA6/Serpin A6/Transcortin/corticosteroid binding globulin/member 6/serpin peptidase inhibitor clade A(alpha-1 antiproteinase antitrypsin) Detection method Sandwich ELISA Double Antibody Application This immunoassay kit allows for the in vitro quantitative determination of SERPINA6 concentrations in serum plasma and other biological fluids. Size 96T Range 39-2500pmol/ml Sensitivity < 22.5pmol/ml Storage 4'C for 6 months Recovery Matrices listed below were spiked with certain level of SERPINA6 and the recovery rates were calculated by comparing the measured value to the expected amount of SERPINA6 in samples. Matrix Recovery range(%) Average(%) serum(n=5) 95-100 99 EDTA plasma(n=5) 89-104 97 UFH plasma(n=5) 92-102 97 Linearity The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SERPINA6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample 1:2 1:4 1:8 1:16 serum(n=5) 85-99% 85-105% 94-101% 86-100% EDTA plasma(n=5) 82-100% 83-100% 85-100% 85-95% UFH plasma(n=5) 82-99% 89-94% 81-97% 82-96% CV(%) Intra-Assay: CV<8% Inter-Assay: CV<10% Note For Research Use Only

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Purchasing Information
Certification RUO
French code (NACRES) NA.84
UNSPSC code 41116126
EU customs code 30021010
Human origin No
Vendor country IE
Brand Assay Genie
Product properties
Supplier category 2 Human ELISA Kit
Supplier category 1 ELISA Genie
Supplier category 3 ELISA
Marketing brochures
Product presentation
Technical presentation
Shelf-Life (months) 06 , 6
Shipping temperature +4°C
Storage temperature +4°C
Applications ELISA

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