PREB Polyclonal Antibody (CAB14347)

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  • 100 μl
CAB14347

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General Information
Description OverviewTitle:PREB Polyclonal Antibody (CAB14347)Size:100 μLCode:CAB14347Host Species:RabbitPurification:Affinity purificationIsotype:IgGBackgroundThis gene encodes a protein that specifically binds to a Pit1-binding element of the prolactin (PRL) promoter. This protein may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p. This gene overlaps the abhydrolase domain containing 1 (ABHD1) gene on the opposite strand. Immunogen InformationImmunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 13-202 of human PREB (NP_037520.1).GeneID:10113SwissProt:Q9HCU5Synonyms:PREB; SEC12; prolactin regulatory element bindingCalculated MW:45kDaObserved MW:50kDaApplicationsReactivity:Human, MouseTested Applications:WBRecommended Dilution:WB 1:500 - 1:2000Storage Buffer:Store at -20 °C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Application Key:Western blotting Positive Samples:U-87MG, A-549, HeLa, LO2, Mouse heartCellular Location:Endoplasmic reticulum membrane, Nucleus, Single-pass membrane proteinAntibody ImagesWestern blot analysis of extracts of various cell lines, using PREB antibody (CAB14347) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s. Datasheets

Product Information

Purchasing Information
Certification RUO
French code (NACRES) NA.41
UNSPSC code 12352203
EU customs code 30021010
Vendor country IE
Human origin No
Target Details
Other names PREB , SEC12 , prolactin regulatory element binding
MW target protein (kDa) 45kDa
Identification
Uniprot Q9HCU5
Brand
Brand Reagent Genie
Product properties
Supplier category 2 Epigenetics and Nuclear Signaling
Supplier category 1 Antibody Genie
Supplier category 3 ANTIBODY
Marketing brochures https://yris.ozyme.fr/reg-brochure
Clonality Polyclonal
Host Rabbit
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 13-202 of human PREB (NP_037520.1).
Isotype (antibody class) IgG
Validated reactivity Human , Mouse
Technical presentation https://www.antibodygenie.com/content/Technical%20Manuals/CAB14347.pdf
KO validated No
Usage
Shelf-life (months) 12
Shipping temperature +4°C
Dilution for application WB 1:500 - 1:2000
Storage temperature +4°C
Applications WB (Western Blot)
Research area Epigenetics and Nuclear Signaling

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