- General Information
- Purchasing Information
- Target Details
- Identification
- Brand
- Product properties
- Usage
- Alternative products
| General Information | |
|---|---|
| Description | OverviewTitle:SPDYA Polyclonal Antibody (CAB13607)Size:100 μLCode:CAB13607Host Species:RabbitPurification:Affinity purificationIsotype:IgGBackgroundN/AImmunogen InformationImmunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human SPDYA (NP_877433.2).GeneID:245711SwissProt:Q5MJ70Synonyms:SPDYA; RINGO3; RINGOA; SPDY1; SPY1; speedy protein ACalculated MW:33kDa/36kDaObserved MW:50kDaApplicationsReactivity:HumanTested Applications:WB; IFRecommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:200Storage Buffer:Store at -20 °C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Application Key:Western blotting Immunofluorescence Positive Samples:A549Cellular Location:NucleusAntibody ImagesWestern blot analysis of extracts of A549 cells, using SPDYA antibody (CAB13607) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 10s.Immunofluorescence analysis of MCF-7 cells using SPDYA antibody (CAB13607). Blue: DAPI for nuclear staining. Datasheets |
Product Information
| Purchasing Information | |
|---|---|
| Old sku | CAB13607 |
| Certification | RUO |
| French code (NACRES) | NA.41 |
| UNSPSC code | 12352203 |
| EU customs code | 30021010 |
| Vendor country | IE |
| Human origin | No |
| Target Details | |
|---|---|
| Other names | RINGO3 , SPDYA , speedy protein A , SPY1 , SPDY1 , RINGOA |
| MW target protein (kDa) | 33kDa/36kDa |
| Identification | |
|---|---|
| Uniprot | Q5MJ70 |
| Brand | |
|---|---|
| Brand | Assay Genie |
| Product properties | |
|---|---|
| Supplier category 1 | Antibody Genie |
| MSDS | https://www.assaygenie.com/content/Antibody%20Genie/CAB/MSDS/CAB%20MSDS.pdf |
| Datasheet/manual | https://www.assaygenie.com/content/Antibody%20Genie/CAB/CAB13607.pdf |
| Marketing brochures | https://yris.ozyme.fr/reg-brochure |
| Supplier category 3 | ANTIBODY |
| Supplier category 2 | Cell Cycle |
| Clonality | Polyclonal |
| Conjugate / tag | Unconjugated |
| Host | Rabbit |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human SPDYA (NP_877433.2). |
| Isotype (antibody class) | IgG |
| Technical presentation | https://www.assaygenie.com/spdya-polyclonal-antibody-cab13607/ |
| Validated reactivity | Human |
| KO validated | No |
| Usage | |
|---|---|
| Shelf-Life (months) | 12 |
| Shipping temperature | +4°C |
| Dilution for application | WB 1:500 - 1:2000 |
| Storage temperature | +4°C |
| Applications | IF (ImmunoFluorescence) , WB (Western Blot) |
| Research area | Cell Cycle |