ZAP70(2F3.2), CF700 conjugate, 0.1mg/mL

Shipped by OZYME

Type SKU Price HT Quantity
  • 100 μl
BTMBNC000811-100
  • 500 μl
BTMBNC000811-500

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General Information
Description ZAP70 is a 70 kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene.In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70 kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis. Primary antibodies are available purified, or with a selection of fluorescent CF&reg; dyes and other labels. CF&reg; dyes offer exceptional brightness and photostability. See the <a href="https://biotium.com/wp-content/uploads/2013/07/CF-Dye-Brochure.pdf" target="_blank" rel="noopener">CF&reg; Dye Selection Guide</a> for more information. Note: Conjugates of blue fluorescent dyes like CF&reg;405S and CF&reg;405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.<div><strong>Stock status:</strong> Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email <a href="mailto:order@biotium.com">order@biotium.com</a> to inquire about stock status and lead times before placing your order.</div>&nbsp; <strong>Catalog number key for antibody number 0811, Anti-ZAP70 (2F3.2)</strong>&nbsp;[table id=253 /]

Product Information

Purchasing Information
Certification RUO
French code (NACRES) NA.44
UNSPSC code 41105331
Human origin No
Target Details
MW target protein (kDa) 70 kDa
Identification
NCBI (gene ID) 7535
Brand
Brand Biotium
Product properties
Supplier category 1 Primary
Clonality Monoclonal
Clone 2F3.2
Concentration 0.1 mg/mL
Conjugate / tag CF700
Host Mouse
Immunogen Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70
Isotype (antibody class) IgG2a, kappa
Validated reactivity Human
Technical presentation https://biotium.com/product/monoclonal-anti-zap70-2f3-2
Usage
Shelf-Life 24
Shipping temperature Room temperature
Dilution for application Immunohistology formalin-fixed 0.5-1 ug/mL
Storage temperature 2 - 8 °C
Applications IF (ImmunoFluorescence) , IHC-Paraffin (paraffin tissues) , FC (FACS)
Research area Immunology

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