Mix & Go! Competent Cells-XJa(DE3) Autolysis (Add 1 ml 500x Arabinose) 10 x 100 μl

Expédié par OZYME

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  • 10 x 100 μl

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General Information

While there are many cell lysis methods available to scientists, unfortunately none of these methods combine all the ideal features for simple, efficient, economical, and gentle lysis of E. coli cells. The E. coli XJ autolysing strains from Zymo Research were engineered to address this problem. Mild expression of a chromosomally encoded bacteriophage λ R gene, encoding the λ lysozyme, also known as λ endolysin, is induced during growth. Cells are harvested intact while the peptidoglycan layer of the cell walls has been protected from digestion by the cytoplasmic membrane. The membrane is, however, amenable to disruption by a brief physico-chemical stress such as a freeze-thaw cycle after harvesting the cells. The XJ Autolysis™ method is highly efficient and takes only minutes (unlike traditional multiple freeze-thaw cycles). It can be applied to any number of samples without increasing processing time and labor (unlike sonication or French-press), is reliable and repeatable (unlike lysozyme treatment), and finally, is fully compatible with a wide range of buffers. Additionally, it does not require use of any potentially interfering components such as detergents, commonly found in various lytic buffers. They are also applicable for nucleic acid purification, and available with a DE3 lysogen encoding the T7 polymerase for expressing recombinant proteins driven by the T7 promoter.

Informations produit

Purchasing Information
Certification RUO
French code (NACRES) NA.55
UNSPSC code 41106501
Country of origin (manufacturing) US
EU customs code 38220000
Vendor country DE
Human origin No
Brand Zymo research
Product properties
Marketing brochures https://files.zymoresearch.com/literature/pdf/b1009-01_e_coli_brochure.pdf
Supplier category 3 Ice Items
MSDS https://files.zymoresearch.com/sds/_t3031_xja-de3-mix-and-go-comp-cells.pdf
Datasheet/manual https://files.zymoresearch.com/datasheets/ds1902_xj_autolysis_e._coli_strains_data_sheet.pdf
Cloning reagents & tools Competent cells
Assay format & throughput Vials , Others
Protein type Recombinant protein
Technical presentation https://www.zymoresearch.com/products/mix-and-go-competent-cells-xjade3-autolysis
Cloning methods Rapid cloning
Shelf-Life (months) 6
Shipping temperature -80°C
Storage temperature -80°C

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